During a public appearance to a cathedral in Staffordshire on the start of the week, the King was shouted at by a protester who asked him about his brother's relationship with convicted sex offender Jeffrey Epstein.
The individual inquired how long the monarch had been known about his sibling's association with Epstein, and also raised doubts about whether he had asked the authorities to "conceal" for Andrew.
Amid the heckling, there were crowds of fans and cheers for the King, who was visiting Staffordshire for an event.
Prince Andrew's connections with Epstein have been under close examination in the past few weeks. The prince, who relinquished his titles earlier this month, has repeatedly denied any wrongdoing.
"How long have you been aware about Prince Andrew and Epstein? Did you request the authorities to cover up for Andrew?"
The King ignored the questions but carried on meeting fans, some of whom shouted "God Save The King". One supporter can be heard asking him for a embrace.
Another woman outside the cathedral was also heard telling the protester to "leave" and to "be quiet".
The controversy over Prince Andrew's connections to Jeffrey Epstein has been revived by sexual abuse claims detailed in Ms. Giuffre's recently released book.
In the memoir, the accuser says she had sexual encounters with the prince on three separate occasions, including one instance with Jeffrey Epstein and "eight other young girls"
Prince Andrew, who agreed to a out-of-court agreement with Ms Giuffre in recent years, has consistently denied any misconduct. In 2019, he told a news program he did not recall meeting her "ever", and that they "never had any sort of sexual contact."
But recently, new inquiries have been asked about how he is able to fund his way of life despite not being a working royal.
The revelation that he has only ever paid a token annual rent on his Windsor mansion has intensified the examination over his living arrangements - even though he made large payments initially, including for improvements.
The Liberal Democrats said he should be summoned before members of parliament to give testimony about his lease of his lodge.
But last Thursday, the authorities refused to give MPs time to debate the prince's status or his home, despite growing pressure for investigation.
The royal household has not issued a statement on whether Prince Andrew might relocate of his home - or where he might reside.
A software engineer and tech writer passionate about AI, cybersecurity, and emerging technologies, with over a decade of industry experience.